Application

Research CategoryEpigenetics & Nuclear Function

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected RRP8 in HeLa, A431, HUVEC, and NIH/3T3 cells.

Anti-RRP8, clone 5C7.1, Cat. No. MABE1013, is a highly specific mouse monoclonal antibody, that targets Ribosomal RNA-processing protein 8 and has been tested for use in Immunocytochemistry and Western Blotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Ribosomal RNA-processing protein 8 (UniProt: O43159; also known as Cerebral protein 1, Nucleomethylin) is encoded by the RRP8 (also known as KIAA0409, NML, hucep-1) gene (Gene ID: 23378) in human. Ribosome biosynthesis is one of the most energy-consuming cellular process, which adapts to changes in intracellular energy status. Ribosomal RNA-processing protein 8 is a novel nuclear protein that serves as an essential component of the energy-dependent nucleolar silencing) complex (eNoSC) that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation or upon elevation of NAD+/NADP+ ratio and activates SIRT1 that leads to deacetylation of histone H3 followed by dimethylation of H3 at Lysine 9 (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus. In the complex, RRP8 binds to H3K9me2 and probably acts as a methyltransferase.

Immunogen

GST-tagged recombinant fragment corresponding to 98 amino acids from the C-terminal half of human Ribosomal RNA-processing protein 8.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein G purified

Format: Purified

Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected RRP8 in HeLa cell lysate.

Specificity

Clone 5C7.1 specifically detects Ribosomal RNA-processing protein 8. It targets an epitope with in the C-terminal half.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~53 kDa observed; 50.72 kDa calculated. Uncharacterized bands may be observed in some lysate(s).